ProScript cDNA Synthesis Kit
Reverse transcription is the process of synthesis of DNA from RNA template. This process is driven by RNA-dependent DNA polymerases, also known as reverse transcriptases. A reverse transcriptase synthesizes a complementary DNA (cDNA) strand from the given RNA template strand aiding to produce cDNA libraries. cDNA libraries contain DNA copies of mRNA from cells and tissues and are used to understand actively expressed genes and their functions at a specific point of time.
ProEnz ProScript cDNA synthesis kit consists of a buffer, dNTPs, Oligo d(T)s, Random hexamers, ProScript M-MuLV Reverse transcriptase and Pro RNase inhibitor which aids in cDNA synthesis. This kit is efficient in conversion of RNA to first strand cDNA, from a wide range of 200ng-1000ng. The RNase inhibitor protects the RNA from degradation. The cDNA produced can be utilized for further amplification and also in real-time gene expression analysis.
Catalog | Reactions | |
---|---|---|
PE2411-10 | 10 | |
PE2411-25 | 25 | |
PE2411-50 | 50 | |
PE2411-100 | 100 |