Pro PCR Kit
Polymerase Chain Reaction, most commonly known as PCR, is a technique based on the ability of DNA polymerase to synthesize complementary DNA strand to the given template strand. It allows amplification of very small sample or a short segment of DNA into millions and billions of copies rapidly. It is the most common and indispensable technique in a research and diagnostic laboratory.
The ProEnz Pro PCR kit consists of 10X PCR reaction buffer with Mg+2 and Pro Taq DNA polymerase enzyme for amplification of the DNA fragment to billions of copies. The PCR buffer is provided with Mg+2 ions to enhance the enzymatic activity of Taq polymerase and boost amplification of PCR. The Taq polymerase incorporates dNTPs in 5’-3’ direction with optimal activity at 72°C.
Taq DNA polymerase is a thermostable enzyme purified from the expression product of E. coli strain that carries the pol A gene from Thermus aquaticus. This unmodified enzyme replicates DNA at 72°C and exhibits half-life at 95°C.
| Catalog | Reactions | |
|---|---|---|
| PE2412 – 10 | 10 | |
| PE2412-25 | 25 | |
| PE2412-50 | 50 | |
| PE2412-100 | 100 | |