Pfu DNA Polymerase Kit
Polymerase Chain Reaction, most commonly known as PCR, is a technique based on the ability of DNA polymerase to synthesize complementary DNA strand to the given template strand. It allows amplification of very small sample or a short segment of DNA into millions and billions of copies rapidly. It is the most common and indispensable technique in a research and diagnostic laboratory.
The ProEnz Pfu polymerase kit consists of 10X Pfu PCR buffer and Pfu polymerase enzyme for amplification of the DNA fragment to billions of copies. The Pfu Polymerase kit is recommended for use in PCR reactions that require high-fidelity synthesis.
Pfu DNA polymerase is a thermostable enzyme of approximately 90 kDa, purified from the expression product of E. coli strain carrying the pol gene from Pyrococcus furiosus. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5’ to 3’ direction, in the presence of Mg2+, creating PCR fragments with blunt ends. It possesses 3’ to 5’ exonuclease (proof-reading) activity and is recommended for use in reactions that require high-fidelity synthesis.
| Catalog | Reactions | |
|---|---|---|
| PE2417-10 | 10 | |
| PE2417-25 | 25 | |
| PE2417-50 | 50 | |
| PE2417-100 | 100 | |